Biology ia

 Biology ia Essay

002141-0012

Nuba Jackson IB Biology

Microbiology IA

How effective is Lysol in the reduction of microbe growth in comparison to Pinesol in reduction of E. Coli growth in agar by room temperatures? п»ї

Background Information:

Pinesol and Lysol are both common household disinfectants that will make very big commercial promises; both claim to kill 99. 9 percent of bacterias. Lysol contains Phenol the industry highly unstable organic compound. Phenol can be described as carbolic acidity found in several household washing supplies. Phenol is very harmful and because of this it is built to kill bacterias. The phenol within the Lysol causes the planet where the bacterias thrive being uninhabitable. Pinesol has other organic substances in that as well but non-e as volatile as Phenol, however some consumers say that Pinesol is just as useful as Lysol. Pinesol is made up of allkyl and isophryll acohol which are both do not affect the environment in the bacteria as effectively.

variables

Way of measuring

range

3rd party

Lysol and pinesol

Concentration

2-3 milliliters

dependant

Bacterial growth

groupe

5-7 Groupe

Controls

Uses

just how

Agar dish

Similar agar enables this specific kind of bacteria to grow; and adds uniformity to environment.

Use same type of agar plates.

dampness

Allows investigator to know how bacteria reacts with condensations

Use same incubator for storage

temperature

Adds regularity to environment and gives bacteria optimum environment for progress.

Use same incubator intended for storage

Sort of bacteria

Adds consistency pertaining to experiment and it is a bacterium

Make use of same sort of bacteria (E. Coli)

Hypothesis:

I do think the Phenol in the Lysol will cause the most significant reduction in bacterial growth, for the reason that Phenol is known as a volatile organic and natural compound capable of bacterial reduction in extensive amounts.

Materials:

•

1 bottle of Lysol

•

you bottle of Pinesol

•

2 managed to graduate cylinders

•

2 bloodstream agar discs

•

sterilized cottonswabs

•

2 incubator fishing rods

•

2 petri dishes

Procedure:

1)

Wash both hands

2)

Packaging the top of the container with the initials and name of household cleaner that is going to provide on the Streptococcus Baccillius.

3)

Begin by sterilizing the petri dishes. Accomplish by simply placing them within a bath of boiling water and allowing to dry upside down on a sanitized blow drying rack or perhaps lab work bench. In the event the Petri meals are still encased in the initial packaging, leave them until willing to use. For 500 milliliters (ml) of agar combination, you can load 25 average-sized petri dishes.

4)

Prepare agar agar powder by simply placing that in the microwave with the suitable amount of water. The label should give specific guidelines although 6th. 9 grms to five-hundred ml of water (or just a little a lot more than two cups) is regular. For agar agar tablets, twelve tablets dissolved in two cups of water can be standard.

5)

Deal with bottled agar formula thoroughly by loosening the bottle cap (but not removing) before positioning in the microwave to nice and soften into a liquid state. Sterilize the container after microwaving by placing the neck with the bottle more than an open flame a few times to make sure any airborne germs happen to be eradicated just before proceeding to plate making.

6)

Place the petri dishes the correct way up and crack nevertheless do not remove the lids. This is certainly to prepare all of them for individual managing when the other hand is holding the agar agar mixture in a suitable pouring vessel such as a glass pitcher.

7)

Gently lift the top of the petri dish with one hand and hover the lid immediately over the underlying part dish to remove any probability of airborne bacteria entering.

8)

Pour an amount of agar liquid in each dish as you hover the cover as mentioned in the previous...